As a vertical autoclave supplier, I get this question all the time: can a vertical autoclave be used for sterilizing surgical gowns? And I understand why it comes up. Surgical gowns aren’t just fabric—they’re a critical part of infection control in operating rooms, where even a tiny bacterial load can lead to post-surgical complications. And vertical autoclaves are one of the most common pieces of sterilization equipment in healthcare facilities, so it makes sense that teams would want to know if they can pull double duty instead of running a separate process. Let’s break this down, not just as someone who sells vertical autoclaves, but as someone who’s worked with clinical teams to make sure their sterilization processes are safe and compliant. Vertical Autoclave

First, I need to ground this in how autoclaves work in the first place. Sterilization via moist heat (the core of autoclaving) relies on three key factors: temperature, pressure, and exposure time. For most medical items, the standard is 121°C (250°F) at 15 PSI for 15 minutes. That combination breaks down microbial cell walls, denatures proteins, and kills everything from bacteria to spores—including the tough Clostridium difficile spores that are a big problem in healthcare settings.
Now, surgical gowns. Modern surgical gowns are made from two main types of fabric: woven, non-woven, or a blend of both, many treated with a hydrophobic barrier to resist liquid penetration. That barrier is what keeps the surgeon sterile during a procedure, but it also means the fabric has to be treated gently enough to preserve that barrier—because if the barrier breaks down, the gown is useless for its intended purpose. Here’s where the vertical autoclave comes in. Horizontal autoclaves (the other common type) are large, designed for bulk items like trays of surgical instruments, but vertical autoclaves are smaller, more compact, often floor-standing or countertop models. They’re perfect for smaller, frequent loads: things like gloves, gowns, masks, or small instrument sets that don’t need a huge chamber.
But here’s the catch: not all vertical autoclaves are the same. Some basic models run only the standard 121°C gravity cycle, while higher-grade units have prevacuum cycles (which pull air out of the chamber first, instead of relying on steam to displace air, which is more effective for porous items). For surgical gowns, prevacuum is non-negotiable. Gravity cycles can leave air pockets in fabric layers, especially if the gown is folded or stacked, so steam can’t reach every part to sterilize it. A prevacuum cycle removes that air, letting steam penetrate the fabric evenly. That’s a detail a lot of people miss when they’re thinking about using a vertical autoclave for gowns—just because it’s a vertical unit doesn’t mean it’s suited for the job.
I’ve seen this play out with a small clinic I worked with last year. They had a basic vertical gravity autoclave, and their team was trying to sterilize reusable cloth surgical gowns (a cheaper alternative to disposable, which is great for small practices cutting costs). The first few runs looked fine, but after a couple of weeks, they had a minor infection scare in a minor procedure room. When we tested the gowns, there were bacteria trapped in the inner layers—turns out the gravity cycle wasn’t penetrating the folded fabric. Once they switched to a vertical autoclave with a prevacuum cycle and adjusted the load (only two gowns per run, no more stacking), the problem went away. That’s not to say basic vertical autoclaves are bad—they’re perfect for instrument trays, glassware, or other dense items where air displacement works—but for porous fabric like surgical gowns, prevacuum is key.
Another thing to consider: load preparation. Surgical gowns can’t be tossed in the chamber any way you want. They need to be hung loosely (vertical autoclaves have hanging racks for exactly this) or laid flat, not folded tight. If you fold a gown into a square, the middle of that fold will stay dry and unsterilized, no matter what cycle you use. I always tell my customers that load preparation is 50% of the sterilization process—even the best vertical autoclave won’t fix a bad load. For surgical gowns, that means taking them out of their packaging, if they’re sterile wraps, hanging them so steam can flow around every inch, and not overloading the chamber. Most vertical autoclaves have a maximum load size, and for gowns, you don’t want to go beyond 60% of the chamber volume. I’ve seen teams try to cram 10 gowns into a 8-liter vertical unit—way too much. That’s a quick way to end up with gowns that aren’t fully sterile.
Now, disposable vs. reusable surgical gowns. This is a big one because a lot of facilities don’t know that vertical autoclaves are mostly used for reusable gowns. Disposable gowns are pre-sterilized at the factory, so you don’t need to reprocess them. Reusable gowns, whether woven polypropylene or spunbond non-woven, are designed to withstand a certain number of autoclave cycles—usually 20 to 50, depending on the fabric. Using a vertical autoclave here is more cost-effective than buying disposable gowns every time, especially for small to medium practices, urgent care centers, or veterinary clinics that don’t do high-volume surgery. But you have to test the gowns after a few cycles to make sure the barrier is still intact. I recommend using a simple barrier test: spray a little water on the outer layer after sterilization—if it absorbs, the gown is no longer a barrier, and it’s time to retire it.
Wait, but what about compliance? Healthcare facilities have to follow standards from organizations like the CDC (Centers for Disease Control and Prevention), WHO (World Health Organization), and AAMI (Association for the Advancement of Medical Instrumentation). The CDC’s guidelines for sterilization and disinfection of reusable medical devices specifically say that porous items like textiles (which surgical gowns are) require prevacuum steam sterilization. So if your vertical autoclave doesn’t have a prevacuum cycle, you can’t use it for surgical gowns in a clinical setting that needs to meet those standards. That’s a non-negotiable point. A lot of low-cost vertical autoclave suppliers market their units as “multi-use” for gowns, but they don’t mention that you need a prevacuum cycle to meet regulatory requirements. I always be transparent about that—no sales pitch should hide a detail that could get a facility cited or lead to patient harm.
I also get asked about time cycles. For surgical gowns, the prevacuum cycle needs to run for at least 10 minutes of exposure time, some guidelines say 15. A basic vertical autoclave gravity cycle might only need 5 minutes for dense items, but that’s not enough for fabric. So even if you have a prevacuum vertical unit, you have to set the cycle to the right duration. I’ve heard of teams shortening cycles to save time, but that’s a mistake. Spending an extra 5 minutes on a sterilization cycle is way cheaper than dealing with a post-surgical infection.
Another factor: temperature. Can you use a lower temperature for gowns? Some facilities use 134°C (273°F) for some heat-sensitive items, but surgical gowns are usually fine at 121°C. 134°C is a higher temperature, which can break down the fabric or barrier faster, reducing the number of times you can reuse a gown. For vertical autoclaves, 121°C is the sweet spot for balancing sterility and gown durability.
Let me share a recent success story to put this in perspective. A regional surgical center in the Midwest was using horizontal autoclaves for both instruments and gowns, but their horizontal units were starting to fail, and replacing them was expensive. They came to me asking if a vertical autoclave could replace the horizontal one for their gown loads, since they only did 15-20 gown sterilization runs a week. We recommended two countertop vertical autoclaves with prevacuum cycles, installed a gown hanging rack, and worked with their staff on load preparation. After three months, they reported a 30% drop in their sterilization-related costs, no issues with gown barrier integrity, and all their infection control checks passed. That’s exactly what I love to see—vertical autoclaves being used for the right job, without cutting corners on safety.
But let’s be honest: there are limitations. If you’re a large hospital with 50+ surgical gowns a day, a vertical autoclave might not be enough. You’d need a larger horizontal autoclave for bulk loads. Also, if your gowns are heat-sensitive (like some coated non-woven gowns), you might need a low-temperature sterilization method (like ethylene oxide) instead of moist heat. But for most small to medium facilities that use reusable surgical gowns, vertical autoclaves work perfectly when used correctly.
One common misconception I hear: “Vertical autoclaves are too small for gowns.” But countertop vertical autoclaves (which are the most common type I sell) have chambers ranging from 5 to 20 liters. That’s more than enough space for 2-4 surgical gowns per run, which is exactly the volume a small practice would need. Floor-standing vertical units can be even larger, up to 50 liters, for facilities that do a bit more volume. The key is matching the unit size to your needs—don’t buy a 5-liter unit if you need to do 10 gowns a day, but a 15-liter vertical will work.
I also want to talk about maintenance. Vertical autoclaves are low-maintenance compared to horizontal units, but they still need regular testing. Every week, you should run a spore test (using Geobacillus stearothermophilus spores, which are resistant to heat) to make sure the cycle is working. For gowns, I recommend testing the barrier after every 10 cycles, as I mentioned earlier. A lot of facilities skip these tests, especially if they’re using smaller vertical units, but that’s how mistakes happen. As a supplier, I always include free training on maintenance and testing when someone buys a vertical autoclave from me—because it’s not just about selling the equipment, it’s about making sure my customers use it safely.
So to answer the original question: yes, a vertical autoclave can be used for sterilizing surgical gowns, but only if three conditions are met. First, the vertical autoclave must have a prevacuum steam cycle (gravity cycles don’t penetrate fabric layers). Second, the gowns are prepared correctly: hung or laid flat, not folded, with enough space for steam flow. Third, you follow the correct time and temperature settings (121°C, 10-15 minutes of exposure, no overloading the chamber). If those boxes are checked, it’s a safe, cost-effective option. If not, you’re risking incomplete sterilization or damaging the gowns’ barrier.
I’ve been a vertical autoclave supplier for 12 years, and I’ve worked with clinics, hospitals, vet practices, and even dental labs. The best part of my job is helping facilities find the right equipment for their specific needs, not just pushing the biggest or most expensive unit. If you’re a facility manager or infection control officer who’s looking to upgrade your sterilization process, or you have questions about whether your current vertical autoclave is suited for surgical gowns, I’m here to help. We can chat through your volume needs, the type of gowns you use, and make sure you’re getting a solution that’s safe and compliant.

Don’t leave your sterilization process to chance. Reach out to me to discuss your requirements.
Rotary Tablet Press References
CDC Guidelines for Sterilization and Disinfection in Healthcare Settings, 2008
AAMI Standard ST79: Comprehensive Guide to Steam Sterilization and Sterility Assurance in Health Care Facilities, 2021
WHO Infection Prevention and Control Guidelines for Surgical Site Prevention, 2016
Textile Barrier Performance for Reusable Surgical Gowns: A Clinical Evaluation, Journal of Hospital Infection, 2022
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